New species of the genus Trichosetodes Ulmer, 1915 (Trichoptera, Leptoceridae) from Ratanakiri province, Cambodia, based on morphological and molecular data.

Three new species of Trichosetodes, namely T.carmelaesp. nov., T.katiengensissp. nov. and T.ratanakiriensissp. nov. are described and illustrated by male specimens. The male genitalia of T.carmelaesp. nov. can be distinguished from the other 16 species of the genus found in Southeast Asia by the shape of the phallicata. The phallicata of T.carmelaesp. nov. bears a tuft of long hairs in the middle of the dorsal edge. Trichosetodeskatiengensissp. nov. can be distinguished from the other species in Southeast Asia by the shape of the phallicata which is divided into dorsal and ventral branches in lateral view, and T.ratanakiriensissp. nov. by the characters of the left inferior appendage and the shape of segment IX. The posterior end of the left inferior appendage of T.ratanakiriensissp. nov. is not forked and the ventral and lateral views of the posteroventral lobes of segment IX are rounded. Illustrations of male genitalia of Trichosetodeskampongspeuensis Malicky & Kong, 2020 are provided for comparison. The molecular diversity of new Trichosetodes species was analyzed using the mitochondrial large subunit ribosomal rRNA gene region (16S rRNA). In terms of their genetic divergence, T.ratanakiriensissp. nov. and T.kampongspeuensis exhibited remarkable proximity, with only a 1.4% distance. On the contrary, T.carmelaesp. nov. displayed genetic disparity exceeding 6.3% when compared to both T.ratanakiriensissp. nov. and T.kampongspeuensis.

Distribution of Ecnomus McLachlan, 1864 (Trichoptera, Ecnomidae) from the Lower Mekong River with a description of Ecnomusstungtrengensis new species.

Background: Trichoptera were surveyed from four different physiographic regions of the Lower Mekong River, including the Northern Highlands, the Khorat Plateau, the Tonle Sap Basin and the Mekong Delta in Myanmar, Laos, Thailand, Cambodia and Vietnam. New information: Twenty-three species of the genus Ecnomus, including a new species, were identified and mapped. Ecnomusmammus Malicky & Chantaramongkol, 1993 is a common species which is found from Tonle Sab Basin and Mekong Delta. Ecnomusalkmene Malicky & Chantaramongkol, 1997, E.volovicus Malicky & Chantaramongkol, 1993 and Ecnomusquordaio Malicky, 1993 are the common species in the area of the Northern Highlands and the Khorat Plateau. Ecnomusplotin Malicky & Laudee 2010 is found in the Mekong Delta. Ecnomustriangularis Sun, 1997 is a new species record for Southeast Asia. In addition, E.stungtrengensis sp. n. is described and illustrated. The male genitalia of E.stungtrengensis sp. n. are clearly different from those of other species in the genus Ecnomus by the shape of superior appendages which are slender and bent downwards distally in lateral view.

Caddisflies from Myanmar: New records and descriptions of new species (Insecta, Trichoptera).

Adult Trichoptera material collected from different localities in Myanmar resulted in the detection of eleven new species which are described as Rhyacophila inwabumensis spec. nov., Hydropsyche kachinenis spec. nov., Limnocentropus rossicorum spec. nov., Lepidostoma pennicularium spec. nov., Lepidostoma indivisum spec. nov., Apatania naumanni spec. nov., Neophylax obliquostriatus spec. nov., Nothopsyche anawrahta spec. nov., Aplatyphylax bamaricus spec. nov., Aplatyphylax myanmaricus spec. nov., and Oecetis kachinomekana spec. nov. Neophylax nigripunctatus Tian Li 1993 (in Tian et al. 1993), syn. nov. is excluded from Neophylax McLachlan 1871 and synonymized with Phylostenax himalus Mosely 1935. Plectrocnemia bifurcata Tian 1993 (in Tian et al. 1993) is synonymized with P. kainam Malicky 1993, syn. nov. Ninety-eight species are recorded from various places in Myanmar including some inadequately known taxa for which new illustrations of the male genitalia are provided.

Three new species of caddisflies (Trichoptera, Hydroptilidae, Polycentropodidae, Leptoceridae) from Khon Phapheng Waterfall, the Mekong River, Laos.

The Mekong River is a hotspot area for freshwater biodiversity, but caddisfly diversity is largely understudied. Three new species of caddisflies from three different families are described and illustrated from Khon Phapheng Waterfall, the Mekong River, Laos; Orthotrichiachoengthongi Malicky & Laudee, sp. nov. (Hydroptilidae), Pseudoneureclipsiskhonphaphengensis Malicky & Thamsenanupap, sp. nov. (Polycentropodidae), and Setodeskarrilai Malicky & Laudee, sp. nov. Orthotrichiachoengthongi Malicky & Laudee, sp. nov. can be differentiated from the most similar OrthotrichiatritonMalicky 2008 by the specific shape of segment X which is long and tubular, pointed apically and curved inward then immediately upward in dorsal view. Pseudoneureclipsiskhonphaphengensis Malicky & Thamsenanupap, sp. nov. differs from the similar P.kaineus Malicky & Bunlue in Malicky et al. 2004 by the shape of the inferior appendages that are usually broad, almost circular in lateral view. Setodeskarrilai Malicky & Laudee, sp. nov. is mainly different to S.omphale Malicky & Changthong in Malicky et al. 2004 by the distal part of segment X which has a brush-like process.

The larva of Rhyacophila albardana McLachlan 1879, including a discriminatory matrix to the Rhyacophila larvae with comb-shaped gills of Austria, Germany and Switzerland (Rhyacophilidae, Trichoptera).

Final instar larvae collected in the Rißbach (Isar catchment, Bavaria) were positively associated with adults of Rhyacophila albardana by barcoding; final instar larvae and adults of this species also were collected at the same time and site in the Lech River (Austria) in the absence of confusing species. These collections and associations enabled a description of the hitherto unknown larva of this species. We present information on the morphology of the larva and illustrate the most important diagnostic features. This dataset is included in a discriminatory matrix of the other larvae with comb-shaped gills (Rhyacophila Hyperrhyacophila Group) known so far from Austria, Germany, and Switzerland. Species can be separated by coloration patterns of the head and prosternum morphology. Rhyacophila albardana is known from Austria, France, Germany, Italy, and Switzerland (Neu et al. 2018).

New species of caddisflies (Trichoptera: Psychomyiidae, Hydropsychidae, Leptoceridae) from Mekong tributaries, Cambodia.

Males of four new species of caddisflies from Kampong Speu and Siem Reap provinces, Cambodia [Psychomyia kimcheangi n. sp. (Psychomyiidae), Macrostemum siemreapensis n. sp. (Hydropsychidae), Leptocerus angkorwatensis n. sp. (Leptoceridae), and Trichosetodes kampongspeuensis n. sp. (Leptoceridae)] are described and illustrated. Psychomyia kimcheangi n. sp. can be distinguished from other species by characters of the inferior appendages. The main body of each inferior appendage has two branches that are separated near its base but are parallel and close together until the end. In Macrostemum siemreapensis n. sp. the dorsal tip of the phallus has no curved hooklet but instead a knot-like protruding structure in ventral view and looped-like structure in dorsal view. Leptocerus angkorwatensis n. sp. can be distinguished from others by the characters of long needled-like preanal appendages. Trichosetodes kampongspeuensis is distinguished from others by the characters of segment IX in ventral view, which is asymmetrical, and inferior appendages that are not divided and asymmetrical.

Four new species of caddisflies (Trichoptera: Polycentropodidae, Psychomyiidae, Hydropsychidae, Odontoceridae) from Khao Nan and Tai Rom Yen National Parks, southern Thailand.

Males of four new species of caddisflies, Polyplectropus hofmaierae n. sp. (Polycentropodidae), Eoneureclipsis chinachotiae n. sp. (Psychomyiidae), Hydropsyche khaonanensis n. sp. (Hydropsychidae), and Lannapsyche tairomyenensis n. sp. (Odontoceridae) are described and illustrated. Polyplectropus hofmaierae n. sp. is distinguished from other species by the shape of the apical end of its inferior appendages and its sharp intermediate appendages. The posterior edges of their inferior appendages run slanting to the ventrodistal point and are densely covered by short and stiff bristles. Eoneureclipsis chinachotiae n. sp. is differentiated by characters of its phallus, as the first two thirds of its length are slender and slightly curved. The distal part has a dorsal hump with a very slender thread on its caudal edge and is slightly bent downward and dilated. Hydropsyche khaonanensis n. sp. can be differentiated from the other species by its phallus, in which the two apicolateral lobes in ventral view have rounded outer edges. Lannapsyche tairomyenensis n. sp. can be separated from the others by characteristics of the dorsal view of segment IX and the harpago of its inferior appendages. Segment IX is triangular with distinctly convex preanal appendages along the posterior edge of this segment, and the harpago and subapical lobes are relatively similar in shape and length.

New species of caddisflies (Trichoptera, Ecnomidae, Polycentropodidae, Psychomyiidae) from Mekong tributaries, Laos.

Four new species of caddisflies are added to the fauna of Mekong River, Laos. Described and illustrated are Ecnomus petchanaae sp. nov. and E. boonsawaengae sp. nov. (Ecnomidae), Polyplectropus proukaewi sp. nov. (Polycentropodidae), and Psychomyia srichanai sp. nov. (Psychomyiidae) from tributaries of the Mekong River, Laos. Ecnomus petchanaae sp. nov. can be distinguished by the characters of the superior appendages, which are slender and club-shaped in lateral view. In E. boonsawaengae sp. nov., the inferior appendages are tubular with a concave incision subapically and truncated apex. Polyplectropus proukaewi sp. nov. has the distal process of the dorsal branch of the inferior appendage close to the base of the inferior appendages and the length of the process is half of inferior appendages. Psychomyia srichanai sp. nov. can be distinguished by the structure of the dorsal branches of the harpagones and apical sclerite. The outer branches of the harpago in the latter species are bifurcated and bent 90° degrees outward, and the inner dorsal branches of harpago are curved outward. The apical sclerite is indiscernible.

The larva of Rhyacophila hartigi Malicky 1971, including a discriminatory matrix to the Italian Rhyacophila larvae with tufted multifilament gills (Rhyacophilidae, Trichoptera).

The collection of adults and larvae sampled at the same site on Sicily and the absence of confusing species at the sampling location enabled a description of the hitherto unknown larva of Rhyacophila hartigi Malicky 1971 (Trichoptera: Rhyacophilidae). We present information on the morphology of the larva and illustrate the most important diagnostic features. This dataset is included in a discriminatory matrix of the ten other Rhyacophila larvae with tufted multifilament gills of Italy described so far. Species can be separated by coloration patterns of head and pronotum, and by anal claw morphology; however, separation of the R. dorsalis-palmeni-simulatrix-vulgaris species quartet is not yet possible. Rhyacophila hartigi is restricted to the southern Apennine Peninsula and the island of Sicily (Cianficconi et al. 2008).

New Caddisflies (Insecta, Trichoptera) from the Krabi and Phang Nga Provinces, southern Thailand.

An updated list of the caddisfly species occurring in Krabi and Phang Nga Provinces, southern Thailand, comprises 76 species. Among them, 69 species are found in Krabi Province and 17 in Phang Nga Province, 4 are new for science and 2 new for Thailand. New species from the families Hydroptilidae (Hydroptila nevdomek sp. n.), Polycentropodidae (Nyctiophylax dosyhpor sp. n.), Psychomyiidae (Psychomyia dabudettak sp. n.), and Leptoceridae (Setodes pokamest sp. n.) are described. Hydroptila namcattien Malicky Chantaramongkol 2007 and Setodes kybele Malicky Chantaramongkol 2006 are new species for Thailand. Faunistic considerations and comparisons to other regions of Thailand are provided.

Discriminatory matrix for the larvae of the European Thremma species (Trichoptera: Thremmatidae).

This synoptic paper is intended to summarize and supplement the information available on the larvae of Thremma McLachlan 1876 in Europe. We present information on the morphology of the larvae and illustrate the most important diagnostic features. This information is used for the construction of a comprehensive discriminatory matrix for the four European species of family Thremmatidae Martynov 1935 known in the larval stage so far. In the context of this matrix, larvae can be easily diagnosed by the shape of mesonotal sclerites, foretrochantins, forefemora and ventral sclerites on abdominal segment I, by head coloration patterns, by case morphology, and by distribution. In addition, ecological characteristics and distributions of the European taxa are briefly discussed.

The larva of Plectrocnemia renetta Malicky 1975 (Trichoptera, Polycentropodidae), including a discriminatory matrix to the larvae of Plectrocnemia Stephens 1836 species of Greece.

This paper describes the previously unknown larva of Plectrocnemia renetta Malicky 1975. Information on the morphology of the final instar larva is given and the most important diagnostic features are illustrated. A preliminary discriminatory matrix for the Greek larvae of Plectrocnemia Stephens 1836 is also provided. Plectrocnemia renetta and P. conspersa conspersa (Curtis 1834) belong to the group where the inner and outer dorsal secondary setae on abdominal segment IX are strongly different in length. These two species can be separated from each other by the arrangement of muscle attachment spots on the head capsule, number and length of setae on abdominal sternum IX, and by distribution patterns. With respect to zoogeography, Plectrocnemia renetta has been reported from Cyprus, Turkey, and from the Greek islands of Ikaria and Samos.

Identification and morphology of a rhyacophilid caddisfly larva from Cyprus: Rhyacophila aphrodite Malicky 1975.

Rhyacophila aphrodite Malicky 1975 is a micro-endemic species of Cyprus and the only rhyacophilid caddisfly recorded on this island. This paper describes the previously unknown larva of this species. Information on the morphology of the final larval instar is given, and the most important diagnostic features are illustrated. In the context of existing Rhyacophilidae identification keys, the larva of R. aphrodite belongs to the group fitted with one single-filament gill on each side of the meso- and metathoraces and one four-filament gill on each side of abdominal segments I to VIII. In addition, a sword process is present on each anal proleg. The distal anal claw tooth is perpendicular to the longitudinal axis of the anal claw and shorter than half of the anal claw width at the distal tooth insertion.

Four new species of Rhyacophila Pictet, 1834 (Trichoptera: Rhyacophilidae) from Southeast Asia.

Four new species of genus Rhyacophila are described and illustrated from Thailand and Myanmar, Southeast Asia. Rhyacophila longicaudata sp. n. is in the R. nigrocephala Group; the very long basal segment of each inferior appendage distinguishes it from other related species. Rhyacophila aksornkoaei sp. n. and R. limsakuli sp. n. are in the R. anatina Group: they can be differentiated from other species of the group by the brush-like parameres and presence of a ventral process of the aedeagus in R. aksornkoaei sp. n., and by the rectangular apical segment of each inferior appendage and the hooked parameres in R. limsakuli sp. n.. Rhyacophila kengtungensis is in the R. yishepa Group and is characterized by the subtriangular shape of the preanal appendages in dorsal view and by the very large dorsal appendages of the phallic apparatus.

The larvae of Limnephilus minos Malicky 1970 and Mesophylax impunctatus aduncus (Navás 1923) (Trichoptera: Limnephilidae), including a discriminatory matrix for the Greek limnephilid larvae with multifilament gills.

Limnephilus minos Malicky 1970 is a micro-endemic caddisfly restricted to the Greek island of Crete. Mesophylax impunctatus aduncus (Navás 1923) is known from Turkey and the southern Balkan peninsula; Greek records range from islands close to the mainland and Skiros to the northern mainland, and Attica. This paper describes the previously unknown larvae of both taxa. Information on the morphology of the 5th larval instar of each taxon is given, and the most important diagnostic characters are illustrated. A discriminatory matrix for the Greek limnephilid larvae with multifilament gills is also provided. In the context of existing identification keys, the larva of L. minos belongs to the group of Limnephilini larvae where face setae are lacking on the mid- and/or hind femora; the species keys together with L. auricula Curtis 1834 and can be separated from the latter species by setae present between the primary setae on the distal section of mid- and hind trochanters. In contrast, face setae are present on the mid- and/or hind femora in M. impunctatus aduncus. Its larva can be easily identified by the fact that 3 or more ventral-edge setae are present on the midfemur, by setae present on both sides of the anal slit, and by its grazer-type mandible lacking terminal teeth.

The larvae of Athripsodes longispinosus longispinosus (Martynov 1909), Athripsodes longispinosus paleochora (Malicky 1972), and Athripsodes bilineatus aegeus Malicky 1999 (Trichoptera: Leptoceridae), including a discriminatory matrix to the larvae of genus Athripsodes Billberg 1820 in Greece.

Larvae of three leptocerid caddisfly subspecies described in the present paper were sampled in Greece and the North Aegean island of Gökceada (Turkey). Information on the morphology of the final larval instar of each is given and the most important diagnostic features are illustrated. The subspecies are integrated into a synoptic discriminatory matrix including the currently known larvae of Greek species of genus Athripsodes. The species can be easily separated by head coloration; the morphology of the ventral apotome; setal and spinal patterns on the metadorsum, foretibiae, and anal prolegs; and by distribution. With respect to distribution, Athripsodes longispinosus longispinosus is known from Bulgaria, the Caucasus area, Turkey, the northern Greek mainland, and the Greek islands of Thasos, Lesbos, Andros, Ikaria, Naxos, and Rhodes. Athripsodes longispinosus paleochora is an endemic of the Greek island of Crete, and A. bilineatus aegeus has been recorded from the Peloponnese; the Greek islands of Euboea, Skiathos, Kithira, Andros; and Turkey.

The larvae of Rhyacophila tsurakiana Malicky 1984, Rhyacophila gudrunae Malicky 1972, and Rhyacophila biegelmeieri Malicky 1984, including an update for the larval key to the Greek species of genus Rhyacophila Stephens 1836 (Rhyacophilidae, Trichoptera).

Based on adults, mature pupae, and larvae sampled at the same sites on mainland Greece and on the Greek islands of Kerkyra (Corfu) and Crete, the previously unknown larval stages of Rhyacophila tsurakiana Malicky 1984, R. gudrunae Malicky 1972, and R. biegelmeieri Malicky 1984 could be associated and identified. This material enabled us to describe and illustrate the final instar larvae of these three species, and to extract reliable diagnostic characters updating the existing larval key to the Greek species of genus Rhyacophila Stephens 1836 (Karaouzas et.al. 2015). In the context of this key, R. tsurakiana (together with R. tristis Pictet 1834 and R. obtusa Klapálek 1894) lacks abdominal gills; the three species can be separated by the shape of the dorsal sclerite of abdominal segment IX and by anal proleg morphology. Rhyacophila gudrunae is the only Greek species so far where larvae are fitted with one four-filament gill on each body side of the abdomen. Finally, R. biegelmeieri has one tufted multifilament gill on each body side and can be separated from four other species sharing this gill morphology by coloration patterns and head widths; however, separation from R. nubila Zetterstedt 1840 and R. palmeni McLachlan 1879 is not yet possible. Rhyacophila tsurakiana and R. biegelmeieri are large-scale endemic caddisfly species of European Ecoregion 6 (Hellenic Western Balkan), whereas R. gudrunae is a micro-endemic species of this region and restricted to the island of Crete (Malicky 2005b; Graf et al. 2008).

Two new species of Caddisflies (Trichoptera: Insecta) from Lower-Hill Evergreen Forests of Southern Thailand.

Two new species of caddisflies, Macrostemum nigralatum n. sp. and Anisocentropus tairomyenensis n. sp., are described and figured from lower-hill evergreen forests in southern Thailand. Wings of M. nigralatum are black, which distinguishes the new species from other related species. Anisocentropus tairomyenensis n. sp. is a member of the subgenus Anisomontropus and is characterized by the obvious basal expansion of the preanal appendages.

Five new species of Caddisflies (Trichoptera: Insecta) from Upper Tenasserim Range, Thailand and Myanmar.

Five new species of caddisflies are described from the Upper Tenasserim Range, including Chimarra keawpradubi n. sp., Ecnomus niyomwasi n. sp., Psychomyia pinsuwanae n. sp., Leptocerus suwannarati n. sp., and Setodes lertpongsombatae n. sp., based on distinctive characters of male genitalia. Chimarra keawpradubi n. sp. differs from other Chimarra spp. in ventral aspect of inferior appendages, each of which is rectangular with a bulging process apicoventrally and with an acute apex. The basal part of each inferior appendage is square and the apical part is narrow in E. niyomwasi n. sp., differentiating it from other species in the genus. Psychomyia pinsuwanae n. sp. differs from P. amphiaraos Malicky and Chantaramongkol 1997, a closely similar species, by characters of the inferior appendages; the inner branch of each inferior appendage has a long, straight spine on its inner side. Leptocerus suwannarati n. sp. can be distinguished from other Leptocerus spp. by the processes of segment X, which are long, thin, and symmetrical. Setodes lertpongsombatae differs from other Setodes spp. in characters of segment X, which is deeply divided subapically into two very long saber-like blades in lateral view.

Larva of Halesus nurag Malicky 1974 (Trichoptera: Limnephilidae) and diagnostic key for the limnephilid larvae of Sardinia.

This paper gives a description of the hitherto unknown larva of Halesus nurag Malicky 1974 (Trichoptera: Limnephilidae). Information on the morphology of the larva is given, and the most important diagnostic features are figured. In the context of the European Halesus species, the larva of H. nurag is morphologically close to H. appenninus Moretti Spinelli Batta 1979, H. digitatus (Schrank 1781), H. radiatus (Curtis 1834), and H. tessellatus (Rambur 1842). This new morphological dataset was used for providing a key to the Sardinian limnephilid larvae. In this context, H. nurag can be separated from the other Sardinian species with single-filament gills described so far by chaetotaxonomical characters, mandible morphology, and details of the lateral protuberance of abdominal segment I.